Agilent Technologies DP235 Guide de l'utilisateur télécharger pdf (Page 167)

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Contents ▲ 167 ▼ Index

• Red to blue

This assay is for applications that apply a red reference dye and analyze blue

fluorescent cells within a red population.

• Checkout Beads

Red checkout beads are loaded into the wells 1, 3 and 5 and blue checkout beads into

the wells 2, 4 and 6. Markers are set according to expected fluorescence levels of the

red and blue beads. The Checkout Beads assay has the properties of a generic assay

(see below).

• siRNA Transfection Viability

Transfection Viability analysis as described in the Application Note: siRNA transfection

optimization with the Agilent 2100 bioanalyzer (Agilent publication number:

5988-9782EN). This assay enables the automatic calculation of transfection efficiency

(TE) in histogram view and viability in transfected cells (ViT) in dot plot view. Required

gating directions and regions are provided as example, but can be adjusted. Final

Transfection Viability (TV) can be calculated by multiplication TE and ViT values derived

from histograms and dot plots.

The settings of predefined assays are optimized to measure the appropriate cell

characteristics. For evaluation, it is only necessary to adjust the markers in histograms or

regions in dot plots.

Predefined assays contain all necessary markers and regions for evaluation. The gating

direction for histograms is given (for details on the gating direction, refer to “Gating” on

page 201). The markers in all samples are connected (changing a marker changes the

corresponding markers in all samples).

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