Agilent Technologies DP235 Guide de l'utilisateur télécharger pdf (Page 580)

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Contents ▲ 580 ▼ Index

NOTE

With RNA assays, you can move the lines marking the start and end points for data

analysis (shown by the long-dashed vertical green lines) which will adjust the entire

baseline for calculation of the area of the total sample.

Baseline Plateau

This setpoint (found in the setpoint explorer) rejects brief, low slope areas such as at

peaks and between non-baseline-resolved peaks. The signal is recognized to be at

baseline whenever the slope of the data is less than the Slope Threshold setpoint (either

positive or negative) for longer than the time set for the Baseline Plateau.

BMP file

BMP is the standard Windows image format. The BMP format supports RGB,

indexed-color, grayscale, and bitmap color modes.

Bubble

If the tip of a pipette is not positioned all the way to the bottom of a well, bubbles can

result (and sometimes bubbles happen even when you are very careful). The vortexing

step that occurs after samples are loaded into the chip is designed to rid the wells of

bubbles and is usually very effective.

If a large bubble is seen at the bottom of a well, remove the sample from the well, pipette

it back in, and continue with the loading procedure.

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Agilent Technologies DP235 Guide de l'utilisateur Page 580

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