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Lab Exercise: Quantification

Building a Calibration Table

12) Now find the last major peak at approximately 3.3 minutes. The compound

name is o-terphenyl and the amount is 2.4 µg /mL. Select the signal that

provides the most abundant area counts. You will also make this a reference

peak.

13) OK this initial Calibration Table to remove lines with zero amounts. This

step will make building your table easier.

14) From the Calibration menu, select Calibration Table to redisplay the

Calibration Table.

15) Save the method.

16) Now, load all signals for data file, Med.d, the medium standard. The

integration will be performed automatically using the integration events

chosen for the last data file.

17) Under the Calibration menu, select Add Level or select the Add new level

from current chromatogram tool. This time, Level 2. OK the box.

18) Fill in the amount fields only. For level 2, Biphenyl is 2.4 µg/mL and the o-

Terphenyl is 7.2 µg/mL.

19) Now load the high-level standard, high.d. Select Add Level. This data file is

for level 3.

20) The amounts for the third level are: Biphenyl, 8.0 µg/mL, and o-Terphenyl,

24 µg/mL.

21) You may click on the chromatographic peak in the chromatogram to view the

corresponding calibration curve in the lower right hand corner. You may

change the curve fit by selecting Peak Details from the Calibration Table

Option Set drop box found in the middle of the window. Now the Calibration

Table contains a Curve Type and Origin column. Select a Linear curve type

and Ignore the origin.

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Agilent Technologies All Guide de dépannage Page 103