Lab Exercise: Quantification
Creating the Acquisition Method
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Note: If a VWD detector is being utilized, select the same wavelength as signal
A.
5) Set the column compartment temperature to 40°C (both left and right). OK
the dialog box.
6) From the Method menu, select Save Method As.... Name the method
Quant.m.
7) Type in a short comment for the Method History and OK.
8) Turn on the pump and equilibrate the HPLC column for several minutes with
30% water/70% acetonitrile.
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