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Lab Exercise: Acquisition Methods

Priming the Agilent 1100/1200 Solvent Delivery System

Priming the Agilent 1100/1200 Solvent Delivery

System

You should prime the pump when you change mobile phases, when the

instrument has been sitting without flow for long periods of time, when you have

performed maintenance on the HPLC, or when you experience periodic

perturbations in the baseline.

1) If you are equipped with a vacuum degasser, make certain the degasser is on.

2) Ensure that the outlet tube is connected from the purge valve to the waste

container. Open the purge valve located on the pump module by turning the

knob counter clockwise several turns.

3) To prime the solvent delivery system, you should pump 100% at 5 mL/min

from each channel for several minutes. To accomplish this task, select the

following menu items: Instrument, Set up Pump...; or click on the pump in

the system diagram (shown below), then Set up Pump....

4) Type in a Flow of 5.000 mL/min and %A of 100. OK the window.

5) To turn the solvent delivery system on, you may use the menu items:

Instrument, More Pump, Control; or, access the tool shown in the diagram

below. Do this now.

Click here

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Agilent Technologies All Guide de dépannage Page 29