Agilent Technologies All Guide de dépannage télécharger pdf (Page 30)

Lab Exercise: Acquisition Methods

Priming the Agilent 1100/1200 Solvent Delivery System

6) Wait until a steady stream of solvent comes out of the purge valve waste tube.

7) Repeat steps 3 through 6 for the other channels of the pump. Change to the

composition for your next run, %B=70.

8) Turn off the pump.

9) Close the purge valve. Set the required composition and flow rate for your

next application. In this case: Flow 1.5 mL/min and %B 70.

10) Turn on the pump and allow the column to equilibrate.

Note: Always keep HPLC grade solvent in all pump channels to prevent valve

damage even when the channel is not being used. For a reversed phase system,

place water with 20% methanol in the unused channels.

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Agilent Technologies All Guide de dépannage Page 30